The Effect of Keluwih (Artocarpus camansi) Leaves Extract On Pro-Inflammatory Expression, Growth Factors and Bodies in Zebrafish Larvae (Danio rerio) Stunting Model

Gondokesumo, Marisca Evalina and Muslikh, Faisal Akhmal (2024) The Effect of Keluwih (Artocarpus camansi) Leaves Extract On Pro-Inflammatory Expression, Growth Factors and Bodies in Zebrafish Larvae (Danio rerio) Stunting Model. Biomedical & Pharmacology Journal, 17 (3). pp. 1647-1653. ISSN 0974-6242; e- ISSN 2456–2610

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Abstract

Stunting is a chronic nutritional problem that occurs during the first 1000 days of life which is considered a golden window of opportunity. Indonesia has the highest prevalence compared to other middle-income countries. Keluwih (Artocarpus camansi) is known to have various compounds that are beneficial for the body such as anti-inflammatory and antioxidant. This study was conducted to determine the potential of the ethanol extract of Keluwih leaves (Artocarpus camansi) zebrafish stunting model against inflammatory markers, growth factors and body size. Artocarpus camansi leaves were extracted using the maceration method for 3x24 hours with 96% ethanol solvent. Zebrafish larvae were obtained from male and female broodstock (2:1), then induced using rotenone and ethanol extract of Artocarpus camansi leaves, then immunohistochemical staining was performed using growh factor (VEGF and TGF-β), inflammation (IL-6 and TNF-α) and body length measurements on day 9 dpf. The results showed that rotenone can provide a picture of stunting in zebrafish larvae from observations of growth factors, inflammation and body length, by administering ethanol extract of Artocarpus camansi leaves this can improve stunting conditions due to administration of rotenone, the concentration of ethanol extract of Artocarpus camansi leaves 2.5 ppm is the optimal concentration in improve stunting conditions. The ethanol extract of Artocarpus camansi leaves can improve stunting conditions by increasing the expression of growth factors, decreasing pro-inflammatory cytokines and improving body length in zebrafish larvae.

Item Type:	Article
Uncontrolled Keywords:	Artocarpus camansi; Stunting, Growth factors; Malnutrition; pro-inflammatory cytokines
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	14 Nov 2024 04:12
Last Modified:	14 Nov 2024 07:36
URI:	http://repository.ubaya.ac.id/id/eprint/47406

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