The Effect of Growth Hormones and Sucrose Concentration on The Growth of Red Ginger (Zingiber Officinale Roxb. Var. Rubrum Rosc.) Callus Culture

Krisnawan, Alfian Hendra and Sukweenadhi, Johan and Rahmadhani, Amelia Violina Caesar Rahmadhani and Syafa, Adrina Mediani and Fitri, Dyana and Marcela, Yeni and Putri, Evanie Noer and Christanti, Pissa and Yunita, Oeke (2024) The Effect of Growth Hormones and Sucrose Concentration on The Growth of Red Ginger (Zingiber Officinale Roxb. Var. Rubrum Rosc.) Callus Culture. In: 3rd International Conference of Biological Science 2024 (ICOBIOS), 28-29 September, Universitas Brawijaya, Malang, Jawa Timur, Indonesia.

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Abstract

Red ginger (Zingiber officinale Roxb. var. rubrum Rosc.) is a medicinal plant widely used for health purposes due to its rich content of the active compound gingerol, with many pharmacological activities. In vitro propagation of ginger can be achieved through the technique of callus culture. This study aimed to determine the optimal growth of red ginger callus on media with different plant growth hormones and sucrose concentrations. The hormones used were 2,4-D at 0.5; 1; 1.5 ppm and NAA at 1; 1.5; 2 ppm with different sucrose concentrations of 3; 4.5; and 6% incubated for 8 weeks. The findings indicate that the most optimal callus diameter growth was achieved by using 0.5 ppm of the 2,4D hormone combined with 3% sucrose concentration, resulting in a 9.00 ± 0.57 mm diameter. Additionally, the combination of NAA at 2 ppm and 3% sucrose concentration also produced a notable callus diameter of 7.33 ± 1.52 mm. These results are expected to provide a solution for the use of hormones and sucrose in the in vitro propagation process of ginger through callus culture.

Item Type: Conference or Workshop Item (Speech)
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy > Department of Pharmacy
Depositing User: Oeke Yunita, S.Si, M.Si. Apt (1161)
Date Deposited: 02 Jan 2025 02:34
Last Modified: 02 Jan 2025 02:34
URI: http://repository.ubaya.ac.id/id/eprint/47575

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