Anticancer Effectivity of Nanocrystals Derived from Mangosteen (Garcinia mangostana) Peel Extract on Leukemia HL-60 Cells

Gondokesumo, Marisca Evalina and Novilla, Arina and Prahastuti, Sijani and Kusuma, Hanna Sari Widya and Widowati, Wahyu and Zahiroh, Fadhilah Haifa and Hadiprasetyo, Dhanar Septyawan and Surakusumah, Wahyu (2025) Anticancer Effectivity of Nanocrystals Derived from Mangosteen (Garcinia mangostana) Peel Extract on Leukemia HL-60 Cells. Science and Technology Indonesia, 10 (1). pp. 228-237. ISSN 2580-4405; e-ISSN 2580-4391

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Abstract

Leukema, characterized by abnormal leukocyte proliferation, ranks ninth in indonesia as the most common cancer. While freatments such as chemotherapy and radation effectively target cancer cells, they also risk damaging healthy blood cells. This has spurred interest in exploring low-toxicity herbal compounds as potential therapies, with mangosteen peel emerging as a widely researched option. Nanotechnology, which has the potential to enhance the bioavailability of herbal compounds, is also a focus of extensive research. This study objective was to assess the impact of Mangosteen Peel Nanocrystal (MPN) on HL-60 leukemia cells by analyzing vanous parameters, including cytotoxicity, reactive oxygen species (ROS) levels, senescence, and gene expression changes. MPN was prepared with high-speed milting and characterized using particle size analyzers, microscopy, and stability assessments. HL-60 cells were cultured and subjected to MPN treatment Cytotoxicity was evaluated using WST-8 assays, ROS levers were assessed using flow cytometry, and senescence analyses using Senescence-Associated b-Galactosidase Staining. AKT and FLT-1 gene expression were determined via qRT- PCR MPN has been successfully characterized as a nanoparticle based on size, stability, and morphology MPN has an impact on leukemia cells by increasing cytotoxicity, decreasing ROS levels, inducing senescence, and modulating AKT and FLT-1 gene expressions The findings suggest potential implications for MPi in targeting leukemia celts. The study sheds light on the promising effects of MPN in leukemia cell models, indicating its potential applications in targeting cancer cells, inducing senescence, decreasing ROS levels, and modulating gene expressions related to cell survival and proliferation.

Item Type:	Article
Uncontrolled Keywords:	Anticancer, Garcinia mangostana, HL-60 Cells, Leukemia, Nanocrystal
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	14 Jan 2025 08:17
Last Modified:	14 Jan 2025 08:17
URI:	http://repository.ubaya.ac.id/id/eprint/47715

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