Jonathan, Jonathan and Suryadjaja, Ernest and Putra, Sulistyo Emantoko Dwi (2023) Bst polymerase enhancement A bioinformatics approach to improve Bst polymerase characteristics. In: Proceedings of the Conference on Natural Resources And Life Sciences 2022 (NRLS-BIO 2022), 24-25 August 2022, Surabaya, Indonesia (Online).
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Abstract
DNA polymerase is a remarkably incredible invention in the biotechnology field. Since its discovery, molecular genetic-based research has been growing rapidly. Various methods for molecular-based diagnostics have been developed since. One of which is Loop-Mediated Isothermal Amplification; this method offers simplicity, sensitivity, and a faster result than conventional PCR. However, the availability of the materials supporting the LAMP reaction is still challenging. LAMP materials are often hard to access in a developing country, and the need to import from other countries significantly increases the price. Here we offer a Bst polymerase design that can be produced with the same or even higher quality than the commercial one. Based on bioinformatics analysis, sto7d fusion to the C-terminus of Bst polymerase (Br512g3.1) shows higher stability and solubility. Sto7d fusion to the C-terminus improves processivity when used in Gss (Bst-like polymerase). Lastly, Sto7d fusion to Br512g3.1 offers higher stability and processivity that can be used to overcome the cost problem of Bst polymerase.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Uncontrolled Keywords: | Polymerase; Bst; POCT; Protein Modification |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Postgraduate Programs > Master Program in Biotechnology |
| Depositing User: | JONATHAN |
| Date Deposited: | 14 Feb 2025 08:32 |
| Last Modified: | 14 Feb 2025 08:32 |
| URI: | http://repository.ubaya.ac.id/id/eprint/48015 |
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