In Vitro α-Glucosidase Enzyme Inhibition Activity Test of Water Extract From Sintok (Cinnamomum sintoc Blume) Bark

Saksono, Astrella Amanda Putri and Simanjuntak, Inana Ratu Soripada and Wahjudi, Mariana and Gondokesumo, Marisca Evalina (2025) In Vitro α-Glucosidase Enzyme Inhibition Activity Test of Water Extract From Sintok (Cinnamomum sintoc Blume) Bark. Pharmaceutical Sciences and Research (PSR), 12 (1). pp. 41-47. ISSN 2407-2354; E-ISSN 2477-0612

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Abstract

Diabetes mellitus is a condition characterized by elevated blood glucose levels beyond the normal limits. One therapeutic approach for managing diabetes mellitus involves inhibiting the α-glucosidase enzyme, which plays a role in glucose absorption in the body. However, the use of oral antidiabetic drugs from the α-glucosidase inhibitor class often causes gastrointestinal side effects. Therefore, exploring natural materials as alternative treatments is a promising option. In Indonesia, one plant with potential as an alternative treatment is sintok (Cinnamomum sintoc Blume), a member of the Cinnamomum genus, which is widely utilized in traditional medicine for chronic diseases such as diabetes mellitus. This study aimed to evaluate the α-glucosidase inhibitory activity of a water extract of sintok bark. Preliminary tests were conducted to determine the optimal conditions for measuring α-glucosidase enzyme inhibition activity in vitro using a UV- vis spectrophotometer. The results showed optimal enzyme activity at a wavelength of 405 nm, an incubation time of 40 minutes, and a substrate concentration of 12.5 mM. The water extract of sintok bark exhibited the highest inhibitory activity at a concentration of 5 ppm, with an inhibition value of 28.66%, while acarbose, used as a positive control, achieved the highest inhibition value of 96.36% at a concentration of 4.5 ppm. In conclusion, the aqueous extract of sintok bark demonstrates inhibitory activity against the α-glucosidase enzyme, indicating its potential as a natural antidiabetic agent.

Item Type:	Article
Uncontrolled Keywords:	acarbose; α-glucosidase; in vitro; sintok; stem bark
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	15 Aug 2025 09:31
Last Modified:	15 Aug 2025 09:31
URI:	http://repository.ubaya.ac.id/id/eprint/49420

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