Isolating and Characterising Chitinolytic Thermophilic Bacteria from Cangar Hot Spring, East Java

Chrisnasari, Ruth and Verina, Devi and Tapatfeto, Aime Clorinda and Pranata, Stefan and Patjajani, Tjandra and Wahjudi, Mariana and Purwanto, Maria Goretti Marianti (2018) Isolating and Characterising Chitinolytic Thermophilic Bacteria from Cangar Hot Spring, East Java. Pertanika Journal Tropical and Agricultural Science , 41 (3). pp. 1437-1448. ISSN 1511-3701. E-ISSN: 2231-8542

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Abstract

In the present study, chitinolytic thermophilic bacteria were collected from Cangar hot spring, East Java, Indonesia and screened. The 16S rRNA gene sequencing was used to identify the isolated bacterium which showed highest chitinolytic activity. The identified isolate was then characterised based on morphological and physiological analyses. The results showed the isolated bacterium belonged to Bacillus licheniformis. This isolate produced large amounts of chitinase on 0.9% (w/v) colloidal chitin (pH 7.0) at 52°C in a very short time (24 hours). Two pairs of primer were designed to detect the presence of glycosyl hydrolase (GH) 18 chitin domain sequences in the isolated bacterium. Two amplicons sized ~250 bp and ~1000 bp were obtained from PCR process. Then the amplicons were sequenced and analysed. The sequencing results showed the isolated Bacillus licheniformis was proven to have genes encoding ChiA and ChiC domain.

Item Type: Article
Uncontrolled Keywords: Bacillus licheniformis, ChiA, ChiC, thermophilic bacteria, thetmostable chitinase
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Ester Sri W. 196039
Date Deposited: 20 Sep 2018 02:36
Last Modified: 05 Nov 2020 05:33
URI: http://repository.ubaya.ac.id/id/eprint/33444

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