INISIASI, OPTIMASI MEDIA DAN PERBANDINGAN PROFIL KROMATOGRAM SENYAWA GOLONGAN TERPENOID, ALKALOID, FLAVONOID KULTUR TUNAS DAN TANAMAN ARAL Gynura Pseudochina (Lour.) DC

Tjie , Kok and H., poppy and W.T., Artadana, Michael and N., Aida (2011) INISIASI, OPTIMASI MEDIA DAN PERBANDINGAN PROFIL KROMATOGRAM SENYAWA GOLONGAN TERPENOID, ALKALOID, FLAVONOID KULTUR TUNAS DAN TANAMAN ARAL Gynura Pseudochina (Lour.) DC. In: Prosiding Seminar Nasional Sains dan Teknologi IV. Lembaga Penelitian Universitas Lampung, Lampung, pp. 733-740. ISBN 978-979-8510-34-2

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Abstract

Gynura pseudochina (Lour.) DC. is among medicinal plants predicted to have anticancer activity, therefore the plant is beneficial to be propagated in the form of tissue cultures. In this research, the inisiation of the culture was performed using Murashige Skoog + kinetin 4 ppm media and the best media for subculturing of the shoot was Murashige Skoog + Benzyl Adenin 3 ppm. In addition, from the research conducted, it was found that the shoot cultures of this plant aged one week prior to stationary phase has produced compounds of terpenoid, alkaloid and flavonoid groups. The terpenoid chromatogram profile of shoot cultures was similar to that of parent plant. It could be seen from the thin layer chromatogram of the extract, giving the same colour after being sprayed with anisaldehydesulfuric acid reagent. The shoot cultures of Gynura pseudochina (Lour.) DC. produced alkaloid compounds, developing orange color when the chromatogram of the extract was treated with Dragendorff, whereas the parent plant had not formed such compounds yet. The chromatogram profile of flavonoid compounds of shoot cultures and parent plant were also similar.

Item Type: Book Section
Uncontrolled Keywords: Shoot cultures, Gynura pseudochina (Lour.) DC., thin layer chromatogram.
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 27 Aug 2014 07:04
Last Modified: 27 Aug 2014 07:04
URI: http://repository.ubaya.ac.id/id/eprint/20379

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