Direct Adventitious Shoots Formation from Tomato Hypocotyls and Cotyledons

Savitri, Wina Dian (2015) Direct Adventitious Shoots Formation from Tomato Hypocotyls and Cotyledons. Proceedings of International Conference NAMES 2015. pp. 77-81. ISSN 2461-0986

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Tomato is consumed by people worldwide as a vegetable or fruit. The improvement of tomato plants through genetic transformation is very important, namely to produce a virus-free tomato stocks. One of the proofs that show the success in the genetic transformation is the favorable outcome on its regeneration. This experiment aimed to find the methods of direct organogenesis from hypocotyl and cotyledon explants of tomato. Tomato seeds were germinated in agar medium with 1 ppm BAP. After two weeks, the hypocotyls and cotyledons were transferred to MS medium with 0.1 ppm TDZ in combination with 1-3 ppm BAP. The results were observed after four weeks of incubation. As many as 25% of adventitious shoots were formed from hypocotyl explants cultured on MS medium supplemented with 0.1 ppm TDZ in combination with 3 ppm BAP. While in cotyledon explants, 62.5% of adventitious shoots successfully formed in MS medium enriched with 0.1 ppm TDZ in combination with 1 ppm BAP.

Item Type: Article
Uncontrolled Keywords: tomato, direct organogenesis, adventitious shoots, TDZ, BAP
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: WINA DIAN SAVITRI
Date Deposited: 26 Sep 2016 06:19
Last Modified: 26 Sep 2016 08:05

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