Preparation of immobilized glucose oxidase wafer enzyme on calcium-bentonite modified by surfactant

Widi, Restu Kartiko and Trisulo, D C and Budhyantoro, Arief and Chrisnasari, Ruth (2017) Preparation of immobilized glucose oxidase wafer enzyme on calcium-bentonite modified by surfactant. IOP Conf. Series: Materials Science and Engineering, 223 (012050). pp. 1-7. ISSN 17578981

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Abstract

Wafer glucose oxidase (GOx) enzymes was produced by addition of PAH (Poly-Allyamine Hydrochloride) polymer into immobilized GOx enzyme on modified- Tetramethylammonium Hydroxide (TMAH) 5%-calsium-bentonite. The use of surfactant molecul (TMAH) is to modify the surface properties and pore size distribution of the Ca-bentonite. These properties are very important to ensure GOx molecules can be bound on the Ca-bentonit surface to be immobilized. The addition of the polymer (PAH) is expected to lead the substrates to be adsorbed onto the enzyme. In this study, wafer enzymes were made in various concentration ratio (Ca-bentonite : PAH) which are 1:0, 1:1, 1:2 and 1:3. The effect of PAH (Poly-Allyamine Hydrochloride) polymer added with various ratios of concentrations can be shown from the capacitance value on LCR meter and enzyme activity using DNS method. The addition of the polymer (PAH) showed effect on the activity of GOx, it can be shown from the decreasing of capacitance value by increasing of PAH concentration

Item Type: Article
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Engineering > Department of Chemical Engineering
Depositing User: Restu Kartiko Widi 6178
Date Deposited: 31 Jan 2018 23:51
Last Modified: 26 Mar 2018 03:33
URI: http://repository.ubaya.ac.id/id/eprint/31554

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