Sukweenadhi, Johan and Kim, Yu-Jin and Yang, Deok-Chun (2017) Transcription Pattern of Catalase Gene from Gynostemma pentaphyllum (Thunb.) Makino during Various Abiotic Stresses. In: NRLS Conference Proceedings International Conference on Natural Resources and Life Sciences (NRLS-2016), 20–21 October 2016, Surabaya.
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Transcription Pattern of Catalase Gene from Gynostemma pentaphyllum (Thunb.) Makino during Various Abiotic Stresses.pdf - Published Version Download (1MB) | Preview |
Abstract
Catalase (CAT) is a group of enzymes that protect cells against oxidative damage generated by reactive oxygen species. A CAT cDNA was previously isolated and characterized from 3-month-old hydroponically cultured Gynostemma pentaphyllum (Thunb.) Makino plants. The ORF is 1 479 bp with a deduced amino acid sequence of 492 residues. CAT from G. pentaphyllum has a molecular mass of 56.97 kDa with an isoelectric point (pI) of 6.95. The temporal expression analysis of leaf samples demonstrated that GpCAT expression could be up-regulated by various environmental stresses such as jasmonic acid electro, oxidative, salt, heavy metal, chilling and heat stress in a certain time period. A three-dimensional structural model of G. pentaphyllum based on its GpCAT cDNA sequence. The temporal expression pattern suggests that the GpCAT could play a role in the molecular defense response of G. pentaphyllum to abiotic stresses.
Item Type: | Conference or Workshop Item (Paper) |
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Uncontrolled Keywords: | Abiotic stress; Catalase; Gene expression; Gynostemma pentaphyllum(Thunb.) Makino. |
Subjects: | Q Science > QK Botany S Agriculture > S Agriculture (General) |
Divisions: | Faculty of Technobiology > Department of Biology |
Depositing User: | JOHAN SUKWEENADHI |
Date Deposited: | 16 Jul 2018 09:01 |
Last Modified: | 30 Mar 2021 04:33 |
URI: | http://repository.ubaya.ac.id/id/eprint/32676 |
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