Molecular Characterization and Expression Analysis of Pathogenesis related Protein 6 from Panax ginseng

Davaajargal, Myagmarjav and Sukweenadhi, Johan and Kim, Yu-Jin and Jang, Moon-Gi and Rahimi, Shadi and Silva, Jennifer and Choi, Jun-Young and Mohanan, Padmanaban and Kwon, Woo-Saeng and Kim, CG and Yang, Deok-Chun (2017) Molecular Characterization and Expression Analysis of Pathogenesis related Protein 6 from Panax ginseng. Russian Journal of Genetics, 53 (11). pp. 1211-1220. ISSN 1022-7954 (Print) 1608-3369 (Online)

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Official URL: https://link.springer.com/article/10.1134/S1022795...

Abstract

Panax ginseng Meyer is one of the important medicinal plants in the world, particularly in Asian countries. Ginseng encounters many stress exposure during its long cultivation period. However, the molecular mechanism of stress resistance is still poorly understood in spite of its importance. In this study, pathogenesis- related protein 6 (PR6), also called proteinase inhibitor (PI), was isolated from ginseng embryogenic callus, named PgPR6. The small size of PR6, containing an open reading frame of 219 bp encoding 72 amino acids, the typical characteristic of PR6 protein, shares the highest sequence similarity to PR6 of Theobroma cacao (69% identity). Sequence and structural analysis indicated that PgPR6 belongs to class Kunitz-type PI family. This is the first report pertaining to the identification of PR6 gene from the P. ginseng genome. The high-level expression of PgPR6 was observed in root as revealed by quantitative real-time PCR. The temporal expression analysis demonstrated that PgPR6 expression was highly up-regulated by signaling molecules, heavy metals, mechanical wounding, chilling, salt, sucrose, and mannitol stress, indicating that PgPR6 may play an important role in the molecular defense response of ginseng to a various range of environmental stresses.

Item Type: Article
Uncontrolled Keywords: Panax ginseng, abiotic stress, biotic stress, pathogenesis-related protein 6, proteinase inhibitor protein, gene expression
Subjects: Q Science > QK Botany
S Agriculture > S Agriculture (General)
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: JOHAN SUKWEENADHI
Date Deposited: 16 Jul 2018 08:44
Last Modified: 16 Jul 2018 08:44
URI: http://repository.ubaya.ac.id/id/eprint/32677

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