DNA THERMAL DENATURATION STUDY OF ANTIOXIDANT COMPOUNDS FROM METHANOL EXTRACT OF Euphorbia humifusa AS POTENTIAL ANTICANCER AGENTS

., Wulansari N. and Purwanto, Maria Goretti Marianti (2012) DNA THERMAL DENATURATION STUDY OF ANTIOXIDANT COMPOUNDS FROM METHANOL EXTRACT OF Euphorbia humifusa AS POTENTIAL ANTICANCER AGENTS. In: International Seminar on Natural Products Medicines : Current Issues on Future Researches and Applications of Natural Product Medicines, 22-23 November 2012, Bandung.

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Abstract

Methanol extract of Euphorbia humifusa posseses cytotoxic effect toward mammaloma cell and high antioxidant activity: EC50 = 56.26±0.66 µg/mL (Heo Seong II, dkk, 2008). This study aims for screening of antioxidant with potential anticancer activity by direct interaction with DNA. Extract of Euphorbia humifusa was obtained by soxhletation with methanol solvent. The extract was concentrated and suspended in water prior to partition with petroleum ether and ethyl acetate. The antioxidant activity of collected fraction of petroleum ether, ethyl acetate. and water was measured to DPPH. The IC50, values found for petroleum ether, ethyl acetate, and water fraction were 91.50 µg/mL; 7.46 µg/mL and 56.18 µpg/mL respectively. The ethyl acetate fraction was subjected to column chromatography on silica gel using chloroform with an increasing volume of methanol as eluent to give eight fractions. The fraction VI and VII showed highest antioxidant activity (30.73% and 66.12% respectively). The fraction VI and VII were further purified on a silica gel column with methanol:acetone:chloroform as gradient eluent. Further purification of fraction VI gave fraction I and fraction L that showed highest antioxidant activity (64.03% and 56.32%). In this research we observed the increase of thermal stability of DNA up to 11°C after mixing with fraction I. The result of phytochemical analysis of fraction I and fraction L indicate that compounds which showed antioxidant activity are alkaloids and essential oils. However, which compound interacts with DNA is still unknown, since purification has not been performed until single component.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Euphorbia humifusa, Antioxidants, DNA recognition, Thermal Denaturation Study
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 08 Jul 2013 01:31
Last Modified: 09 Jul 2013 05:25
URI: http://repository.ubaya.ac.id/id/eprint/3492

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