Screening Senyawa Anti Kanker Ekstrak Typhonium Flagelliforme (Keladi Tikus) Yang Berinteraksi Dengan DNA

Purwanto, Maria Goretti Marianti and Kok, Tjie and Melinda, Ratna (2011) Screening Senyawa Anti Kanker Ekstrak Typhonium Flagelliforme (Keladi Tikus) Yang Berinteraksi Dengan DNA. Jurnal Ilmiah Sains & Teknologi. ISSN 0216-1540

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Research was conducted in order to detect active chemical constituent of Typhonium flagelliforme which has antioxidant property and at once, also able to interact with DNA. Active extract was determined by evaluation of antioxidant activity using DPPH. The most active extract of Typhonium flagelliforme was further purified by column chromatography. Active fraction is partially analyzed and visualized at 365 nm and sprayed by visualization reagent to detect alkaloids, terpenoids and flavonoids qualitatively. The capability of interaction with DNA was investigated by thermal denaturation assay. The result showed that highest antioxidant activity is given by dichloromethane extract with an IC50 = 245.989 ppm. The most active fraction was obtained from the root with an IC50=231.199 ppm. Thermal denaturation assay showed interaction between DNA and constituent in most active fraction. Alteration in DNA melting temperature (Tm) indicated interaction between DNA and the constituents. More than one active constituent was indicated by TLC analysis. Phytochemistry analysis showed that the most active fraction contains alkaloids, terpenoids and flavonoids.

Item Type: Article
Uncontrolled Keywords: Typhonium flagelliforme, IC50,Tm, DNA
Subjects: Q Science > QD Chemistry
R Medicine > R Medicine (General)
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 08 Jul 2013 04:23
Last Modified: 16 Oct 2020 04:08

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