PRELIMINARY STUDY ON ANTIOXIDANT PROPERTY OF TYPHONIUM FLAGELLIFORME AND ITS INTERACTION WITH DNA

Purwanto, Maria Goretti Marianti and Tjie , Kok and Melinda, Ratna (2011) PRELIMINARY STUDY ON ANTIOXIDANT PROPERTY OF TYPHONIUM FLAGELLIFORME AND ITS INTERACTION WITH DNA. In: Humboldt Kolleg: SYNERGY, NETWORKING AND THE ROLE OF FUNDAMENTAL RESEARCH DEVELOPMENT IN ASEAN in Conjuction with THE INTERNATIONAL CONFERENCE ON NATURAL SCIENCES (ICONS) 2011 (Conference Program and Abstract). Universitas Ma Chung, Malang.

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Abstract

The research was conducted in order to detect active chemicals of Typhoniumflagelliforme with antioxidant property and at the same time it is also able to interact with DNA. The activity of each extract was determined by evaluation of antioxidant activity using DPPH reagent. The most active extract of Typhoniumflagelliforme was further purified by a column chromatography. The result showed that the highest antioxidant activity was given by dichloromethane extract of the root with IC50= 245.989 ppm. The capability of interaction with DNA was investigated by Thermal Denaturation Assay. Changes in DNA melting temperature (Tm) indicated the interaction between DNA and the constituents of the most active fraction. There are more than one active constituent indicated by TLC analysis. TLC spots were visualized at 365 nm and sprayed by visualization reagent to detect alkaloids, terpenoids and flavonoids qualitatively. Phytochemistry analysis showed that the most active fraction of Typhoniumflagelliforme possibly contains alkaloids, terpenoids and flavonoids.

Item Type: Book Section
Uncontrolled Keywords: Typhoniumflagelliforme DNA recognition, Tm, DNA
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 08 Jul 2013 06:50
Last Modified: 09 Jul 2013 05:11
URI: http://repository.ubaya.ac.id/id/eprint/3498

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