IN VITRO PRODUCTION OF SECONDARY METABOLITES FROM GYNURA PSEUDOCHINA (Lour.) D.C.

Purwanto, Maria Goretti Marianti and Rijanto, Anna and Artadhana, I.B. and Tjie , Kok and Theodorus, Selvy and Tanojo, Michael William (2010) IN VITRO PRODUCTION OF SECONDARY METABOLITES FROM GYNURA PSEUDOCHINA (Lour.) D.C. In: Proceedings International Conference on Medicinal Plants in Occasion of the 38th. Meeting of National Working Group on Indonesia Medicinal Plant. Faculty of Pharmacy Widya Mandala in Collaboration with National Working Group on Indonesian medical Plant and German Academic Exchange Service, Surabaya, pp. 91-100. ISBN 978-602-96839-1-2

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Abstract

Gynura pseudochina (Lour.) D.C., or commonly called "Daun Dewa" in Indonesian language, is a local medicinal plant believed to be able to treat cancer and some other diseases. Many researchers have been studying its secondary metabolites and some compounds have been identified. From the production point of view, it is also interesting to consider whether or not its in vitro culture will give the same spectrum of secondary metabolite. Growth curve of in vitro Gynura pseudochina (Lour.) D.C. showed that exponential phase was achieved after three weeks while stationer phase has been started right four weeks (MS media + BA 3%) after inoculation. Samples were, therefore, taken after three and four weeks, respectively, to study the profile of their secondary metabolite. Extraction was carried over some degree of solvent polarities and separated compounds were visualized using W light at 254 and 365 nm. Additionally, qualitative analysis of the compounds was performed using specific spray reagents for flavonoids, terpenoids and alkaloids, respectively. While terpenoid content seemed to be very similar between that produced by in vitro and external plant, there were some evidence on differences of flavonoids and alkaloids content between in vitro plant and external one. In general, in vitro culture has been observed to produce higher amount and variety of secondary metabolites both in three week- and four week- samples.

Item Type: Book Section
Uncontrolled Keywords: in vitro Gynura pseudochina (Lour.) D.C., secondary metabolite.
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 08 Jul 2013 07:02
Last Modified: 22 Aug 2020 15:33
URI: http://repository.ubaya.ac.id/id/eprint/3499

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