Silva, Jeniffer and Sukweenadhi, Johan and Myagmarjav, Davaajarga and Mohanan, Padmanaban and Yu, Junping and Shi, Jianxin and Jung, Ki Hong and Zhang, Dabing and Yang, Deok-Chun and Kim, Yu-Jin (2020) Overexpression of a novel cytochrome P450 monooxygenase gene, CYP704B1, from Panax ginseng increase biomass of reproductive tissues in transgenic Arabidopsis. Molecular Biology Reports, 47 (6). pp. 4507-4518. ISSN 0301-4851 (print) 1573-4978 (web)
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Abstract
Cytochrome P450 monooxygenase 704B (CYP704B), a member of the CYP86 clan, was found to be needed in Arabidopsis and rice to biosynthesize precursors of sporopollenin through oxidizing fatty acids. In the present study, we cloned and characterized a CYP704B gene in Panax ginseng, named PgCYP704B1. It shared high sequence identity (98–99%) with CYP704 of Arabidopsis, Theobroma cacao, and Morus notabilis. The phylogenetic comparison of ginseng and higher plants between the members of CYP86 clan revealed that ginseng CYP704 was categorized as a group of CYP704B with dicot plants. The expression of PgCYP704B1 is low in the stem, leaf, and fruit, and high in fower buds, particularly detected in the young gametic cell and tapetum layer of the developing anther. Arabidopsis plants overexpressing PgCYP704B1 improved plant biomass such as plant height, siliques and seed number and size. A cytological observation by transverse and longitudinal semi-thin sections of the siliques cuticles revealed that the cell length increased. Furthermore a chemical analysis showed that PgCYP704B1ox lines increased their cutin monomers contents in the siliques. Our results suggest that PgCYP704B1 has a conserved role during male reproduction for fatty acid biosynthesis and its overexpression increases cutin monomers in siliques that eventually could be used for seed production
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Panax ginseng; Cytochrome P450; Overexpressing plants; PgCYP704B1; Reproduction; Fatty acid; Seed yield; Biomass |
| Subjects: | Q Science > QH Natural history > QH301 Biology S Agriculture > SB Plant culture |
| Divisions: | Faculty of Technobiology > Department of Biology |
| Depositing User: | JOHAN SUKWEENADHI |
| Date Deposited: | 07 Jan 2021 03:04 |
| Last Modified: | 09 Feb 2022 07:36 |
| URI: | http://repository.ubaya.ac.id/id/eprint/38726 |
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