Critical Aspects to Produce Low-Cost Protein Molecular Weight Marker : A Review

Gunawan, Leonardo Tedjo and Purwanto, Maria Goretti Marianti (2020) Critical Aspects to Produce Low-Cost Protein Molecular Weight Marker : A Review. Indonesian Journal of Biotechnology and Biodiversity 118-127, 4 (2). pp. 118-127. ISSN 9772657140002

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Abstract

Proteomic studies usually begin with characterizing protein profile, such as the molecular weight of protein, which can be done by the SDS-PAGE technique followed by Western Blot analysis. These methods need a standard protein called molecular weight marker (MWM). In this review, the important, fundamental aspects of either recombinant or native MWM production were discussed, including the type and effect of dyes used to prepare a prestained MWM. Moreover, buffer and polyols used in the formulation can also affect the quality and stability. Also, another adjuvant may be needed to increase the robustness of MWM to lower the risk of protease contamination that can breakdown the protein inside the MWM during storage. Understanding those critical aspects will help to produce or formulate a good quality of MWM.

Item Type:	Article
Uncontrolled Keywords:	protein ladder, protein stability, electrophoresis
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	Maria Goretti Marianti Purwanto
Date Deposited:	11 Jan 2021 03:45
Last Modified:	25 May 2022 03:00
URI:	http://repository.ubaya.ac.id/id/eprint/38732

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