Evaluasi Pertumbuhan Suspensi Sel Dendrobium anosmum var. gigantea dan Aktivitasnya sebagai Antioksidan

Prasetyo, Yanuar and Njudang, Kristian and Wibowo, Handriyanto Mulyono Putra and Krisnawan, Alfian Hendra (2020) Evaluasi Pertumbuhan Suspensi Sel Dendrobium anosmum var. gigantea dan Aktivitasnya sebagai Antioksidan. Media Pharmaceutica Indonesiana, 3 (2). pp. 70-79. ISSN 1411-8734

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Abstract

Dendrobium is an orchid plant that has health benefits and included in traditional Chinese medicine. Dendrobium anosmum var. gigantea can be developed using the plant tissue culture method because it has totipotential properties. The growth of cell suspension cultures can be influenced by environmental conditions and the composition of the culture media. Optimization is needed so that optimal growth can be obtained. This study was conducted to see the effect of mass concentration in a flash shaker (10 mg / 10 ml; 10 mg / 20 ml; and 10 mg / 30 ml) and the concentration of sucrose media in the bioreactor (30 g/l; 35 g/l; 40 g/L) on the growth of Dendrobium anosmum var. gigantea cell suspension. Phytochemical screening and antioxidant activity tests were carried out to determine the class of compounds and extract activity as antioxidants. The media used is enriched using the growth hormone NAA (Naphthalene Acetic Acid) : BAP (Benzyl Amino Purine) 0.5 ppm : 0.5 ppm. Phytochemical screening was carried out using the spot test method. Antioxidant activity was tested using the DPPH method. The results showed that the growth of cell suspension on a flash shaker with a concentration of 10 mg / 30 ml and a bioreactor with 35 g/l sucrose showed the most optimal results. The antioxidant activity of the cell suspension culture has a better IC50 value than the parent plant, namely 53,930 ppm, but it is still categorized as very weak.

Item Type:	Article
Uncontrolled Keywords:	Antioksidan; Bioreaktor; Dendrobium anosmum var. gigantea; Kultur suspensi selAntioksidan; Bioreaktor; Dendrobium anosmum var. gigantea; Kultur suspensi sel
Subjects:	Q Science > QK Botany S Agriculture > SB Plant culture
Divisions:	Faculty of Engineering > Department of Product Design and Management
Depositing User:	ALFIAN HENDRA KRISNAWAN
Date Deposited:	12 Jan 2021 07:33
Last Modified:	01 Sep 2021 06:49
URI:	http://repository.ubaya.ac.id/id/eprint/38743

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