Validating and developing TLC-based fingerprinting for Curcuma longa L.

Kartini, Kartini and Jayani, Nikmatul Ikhrom Eka and Hadiyat, Mochammad Arbi (2021) Validating and developing TLC-based fingerprinting for Curcuma longa L. Journal of Pharmacy & Pharmacognosy Research, 9 (5). pp. 704-715. ISSN 0719-4250

PDF Kartini_Validating and developing TLC-based fingerprinting for Curcuma.pdf Download (2MB)

Abstract

Context: Curcuma longa (turmeric) is extensively cultivated as spices and herbal medicines in tropical and sub-tropical countries. Geographical origin is known to significantly determine the quality of the herbs used and, consequently, the safety and efficacy of their products. Aims: To validate and develop TLC-fingerprint combined with chemometrics to differentiate C. longa collected from various origins. Methods: Thin Layer Chromatography (TLC) was employed together with chemometric methods, i.e., Principal Component Analysis (PCA) and Cluster Analysis (CA), to evaluate the quality of C. longa rhizomes collected from nine origins in Indonesia. Results: Chloroform, dichloromethane, and ethanol (64:64:1) were a suitable mobile phase for C. longa. The method used met the requirements for a stable and precise TLC system. As analyzed by the chemometric techniques, the TLC-fingerprints could discriminate C. longa from various origins. The PCA score plot of the first two principal components (PCs) and CA clearly distinguished two clusters of simples. Conclusions: When combined with PCA and CA, TLC-fingerprinting can discern the rhizomes of C. longa sourced from various locations. TLCfingerprints that are analyzed with chemometrics can be used as an alternative marker-oriented method for evaluating the quality of C. longa.

Item Type:	Article
Uncontrolled Keywords:	cluster analysis;geographical origin; herbal medicine; principal component analysis; quality
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Kartini
Date Deposited:	24 May 2021 06:51
Last Modified:	27 Sep 2021 04:51
URI:	http://repository.ubaya.ac.id/id/eprint/39556

Actions (login required)

View Item