Evaluation of Apium graveolens from different geographical origins based on TLC-fingerprint and chemometrics

Kartini, Kartini and Oktaviyanti, Nina Dewi and Setiawan, Finna and Jayani, Nikmatul Ikhrom Eka and Jannah, Miftahul and Wulandari, Fitria (2021) Evaluation of Apium graveolens from different geographical origins based on TLC-fingerprint and chemometrics. In: 4th International Conference on Bioscience and Biotechnology, 21st-23rd September 2021, Virtual.

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Abstract

Apium graveolens (celery) has various roles both in the food and medicine sectors. It grows very well in the tropical and subtropical areas of Africa and Asia, including Indonesia. This Apiaceae member contains a number of phytoconstituents, and geographical origin is known to significantly determine the type and concentration of phytochemicals in plant material. This study was carried out to validate and develop thin layer chromatography (TLC)- based fingerprinting combined with chemometrics, i.e., Principal Component Analysis (PCA) and Cluster Analysis (CA), to evaluate the quality of celery harvested from thirteen different geographical origins in Indonesia. The mobile phase was first optimized with a simplex axial design, resulting in 2-propanol, toluene, and dichloromethane (1:6:1) as the optimum mobile phase for a stable and precise TLC system in the celery sample analysis. When analyzed with chemometrics, the TLC-fingerprints could discriminate celeries from various origins. The PCA score plot of the first two principal components (PCs) and CA clearly distinguished the samples’ properties and classified them into four clusters. Samples grouped into one cluster were concluded to have comparable quality, while those in different clusters had different qualities.

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	pium graveolens, celery, cluster analysis, geographical origins, herbal quality, Principal Components Analysis
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Kartini
Date Deposited:	06 Dec 2021 07:10
Last Modified:	03 Jun 2022 08:32
URI:	http://repository.ubaya.ac.id/id/eprint/40793

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