Overexpression of the Panax ginseng CYP703 Alters Cutin Composition of Reproductive Tissues in Arabidopsis

Kim, Jihyun and Silva, Jeniffer and Park, Chanwoo and Kim, Younghun and Park, Nayeon and Sukweenadhi, Johan and Yu, Junping and Shi, Jianxin and Zhang, Dabing and Kim, Keun Ki and Son, Hong-Joo and Park, Hyeon Cheal and Hong, Chang-Oh and Lee, Kwang Min and Kim, Yu-Jin (2022) Overexpression of the Panax ginseng CYP703 Alters Cutin Composition of Reproductive Tissues in Arabidopsis. Plants, 11 (383). pp. 1-15. ISSN 22237747

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Official URL / DOI: https://doi.org/10.3390/plants11030383


Cytochrome P450 (CYP) catalyzes a wide variety of monooxygenation reactions in plant primary and secondary metabolisms. Land plants contain CYP703, belonging to the CYP71 clan, which catalyzes the biochemical pathway of fatty acid hydroxylation, especially in male reproductive tissues. Korean/Asian ginseng (Panax ginseng Meyer) has been regarded as one of important medicinal plant for a long time, however the molecular mechanism is less known on its development. In this study, we identified and characterized a CYP703A gene in P. ginseng (PgCYP703A4), regarding reproductive development. PgCYP703A4 shared a high-sequence identity (81–83%) with predicted amino acid as CYP703 in Dancus carota, Pistacia vera, and Camellia sinensis as well as 76% of amino acid sequence identity with reported CYP703 in Arabidopsis thaliana and 75% with Oryza sativa. Amino acid alignment and phylogenetic comparison of P. ginseng with higher plants and known A. thaliana members clearly distinguish the CYP703 members, each containing the AATDTS oxygen binding motif and PERH as a clade signature. The expression of PgCYP704B1 was only detected in P. ginseng flower buds, particularly in meiotic cells and the tapetum layer of developing anther, indicating the conserved role on male reproduction with At- and Os- CYP703. To acquire the clue of function, we transformed the PgCYP703A4 in A. thaliana. Independent overexpressing lines (PgCYP703A4ox) increased silique size and seed number, and altered the contents of fatty acids composition of cutin monomer in the siliques. Our results indicate that PgCYP703A4 is involved in fatty acid hydroxylation which affects cutin production and fruit size.

Item Type: Article
Uncontrolled Keywords: cytochrome P450; reproductive tissues; PgCYP703A4; fatty acid; reproduction; Panax ginseng
Subjects: Q Science > Q Science (General)
Q Science > QK Botany
S Agriculture > S Agriculture (General)
Divisions: Faculty of Technobiology > Department of Biology
Date Deposited: 08 Feb 2022 08:27
Last Modified: 08 Feb 2022 08:27
URI: http://repository.ubaya.ac.id/id/eprint/41207

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