Lycopene Content In Several Ages Of Tomato Callus (Lycopersicon esculentum Mill. cv. Rampai)

Goenawan, Yohanes Alvin and Khodjojo, Katherine Krisyanti and Hardjo, Popy Hartatie and Purwanto, Maria Goretti Marianti (2022) Lycopene Content In Several Ages Of Tomato Callus (Lycopersicon esculentum Mill. cv. Rampai). AGRIC Journal of Agricultural Science, 34 (1). pp. 89-104. ISSN 2549-9343

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Abstract

Tomato (Lycopersiconesculentum Mill. cv. Rampai) is rich in lycopene, a carotenoid group compound with many biological activities, one of which is as an antioxidant. Conventional tomato cultivation is not optimal due to the susceptibility of tomato plants to pests and diseases, even though the need for lycopene continues to increase. Plant tissue culture method can be one solution for lycopene production. Callus tomato cv. Rampai was initiated from cotyledons on MS medium (Murashige& Skoog) with the addition of 1 ppm BA (benzyladenine) and 1.5 ppm IAA (indole-3-acetic acid). Callus aged one week to four weeks were harvested and lycopene was extracted by maceration method using hexane:acetone (9:1) as solvent. The presence of lycopene in callus was confirmed by TLC (thin-layer chromatography) (retention factor value/Rf = 0.56) and UV-Vis spectrophotometry (wavelength that gives maximum absorbance = 472 nm). Lycopene content in tomato callus increased with increasing callus age and was directly proportional to the callus growth index. The highest lycopene level was found in callus aged 4 weeks, which was 0.3094 mg/100 g dry weight callus.

Item Type:	Article
Uncontrolled Keywords:	carotenoid, plant tissue culture, thin-layer chromatography (TLC), UV-Vis spectrophotometry
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	Yohanes Alvin Goenawan
Date Deposited:	11 Aug 2022 06:36
Last Modified:	24 Jul 2024 09:23
URI:	http://repository.ubaya.ac.id/id/eprint/42302

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