Thymoquinone Modulates Local MMP-9, IL-10, and IgG in Sciatic Nerve Crush Injury Animal Model

Besin, Valentinus and Bajamal, Abdul Hafid and Sudiana, I Ketut and Agil, Mangestuti and Nugraha, Jusak and Machfoed, Mohammad Hasan and Notopuro, Paulus Budiono and Naesilla, Naesilla (2022) Thymoquinone Modulates Local MMP-9, IL-10, and IgG in Sciatic Nerve Crush Injury Animal Model. The Indonesian Biomedical Journal, 14 (3). pp. 316-322. ISSN 2085-3297, Online ISSN: 2355-9179

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Abstract

BACKGROUND: Interleukin (IL)-10 is involved in Wallerian degeneration after peripheral nerve crush injury. Oral thymoquinone was previously observed to decrease local immunoglobulin-G (IgG) in a crush- injured rat model. No study has evaluated the pathway of various thymoquinone dosages on local IgG and IL-10 in this injury. METHODS: This experimental study used 126 Rattus norvegicus Wistar rats that were divided into 18 groups: six groups received a placebo, the other six groups received thymoquinone at 100 mg/kg/day and the last six groups received thymoquinone at 250 mg/kg/day, respectively. Rats were sacrificed at 12, 18, 24, 5x24, 6x24, and 7x24 hours. Matrix metalloproteinase-9 (MMP-9), IL-10, and local IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). The nuclear factor KappaB (NF-κB) expressions on Schwann cells were examined by flow cytometry. Path analysis was performed using SmartPLS. RESULTS: The path analysis showed that 100mg/kg/day of thymoquinone significantly decreased NF-κB expression. However, NF-κB did not affect local MMP-9, and MMP- 9 had no significant relationship with local IL-10 and IgG. Thymoquinone 250 mg/kg/day also significantly inhibited NF-kB expression, decreased local MMP-9, and, in turn, decreased local IL-10 and IgG. CONCLUSION: Administration of oral thymoquinone 250 mg/kg/day decreases local IgG and IL-10 levels via suppressing NF-κB expression and MMP-9 levels

Item Type:	Article
Uncontrolled Keywords:	thymoquinone, crush injury, IgG, IL-10, MMP-9, NF-κB
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medical
Depositing User:	Ester Sri W. 196039
Date Deposited:	12 Sep 2022 06:16
Last Modified:	30 Mar 2023 05:15
URI:	http://repository.ubaya.ac.id/id/eprint/42526

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