Bacterial lipoxygenases: Biochemical characteristics, molecular structure and potential applications

Chrisnasari, Ruth and Hennebelle, Marie and Vincken, Jean-Paul and Berkel, Willem J.H. van and Ewing, Tom A. (2022) Bacterial lipoxygenases: Biochemical characteristics, molecular structure and potential applications. Biotechnology Advances, 61. p. 108046. ISSN Online 1873-1899, Print ISSN 0734-9750

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Lipoxygenases (LOXs) are enzymes that catalyze dioxygenation of polyunsaturated fatty acids into fatty acid hydroperoxides. The formed fatty acid hydroperoxides are of interest as they can readily be transformed to a number of value-added compounds. LOXs are widely distributed in both eukaryotic and prokaryotic organisms, including humans, animals, plants, fungi and bacteria. Compared to eukaryotic enzymes, bacterial enzymes are typically easier to produce at industrial scale in a heterologous host. However, many bacterial LOXs were only identified relatively recently and their structure and biochemical characteristics have not been extensively studied. A better understanding of bacterial LOXs' structure and characteristics will lead to the wider application of these enzymes in industrial processes. This review focuses on recent findings on the biochemical characteristics of bacterial LOXs in relation to their molecular structure. The basis of LOX catalysis as well as emerging determinants explaining the regio- and enantioselectivity of different LOXs are also summarized and critically reviewed. Clustering and phylogenetic analyses of bacterial LOX sequences were performed. Finally, the improvement of bacterial LOXs by mutagenesis approaches and their application in chemical synthesis are discussed.

Item Type: Article
Uncontrolled Keywords: Enantioselectivity; Fatty acid; Lipoxygenase; Oxidoreductase; Oxidation; Phylogeny; Regioselectivity
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: RUTH CHRISNASARI - 210001
Date Deposited: 20 Mar 2023 07:36
Last Modified: 27 Mar 2023 08:02

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