Modulating dioxygenase and hydroperoxide isomerase activities in Burkholderia thailandensis lipoxygenase

Chrisnasari, Ruth and Hilgers, Roelant and Li, Guanna and Vincken, Jean-Paul and van Berkel, Willem J. H. and Hennebelle, Marie and Ewing, Tom A. (2025) Modulating dioxygenase and hydroperoxide isomerase activities in Burkholderia thailandensis lipoxygenase. Enzyme and Microbial Technology, 191. 110709/1-11. ISSN 1879-0909

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Abstract

Lipoxygenases (LOXs) are enzymes that catalyze the regioselective dioxygenation of polyunsaturated fatty acids (PUFAs), leading to the formation of fatty acid hydroperoxides (FAHPs). In addition to dioxygenase activity, some eukaryotic LOXs exhibit hydroperoxide isomerase (HPI) activity under specific conditions, resulting in the production of structurally diverse compounds such as epoxy alcohols and ketones. Until now, the presence of HPI activity in bacterial LOXs has not been documented. In this study, we investigated the HPI activity of LOX from Burkholderia thailandensis (Bt-LOX) and examined the effects of reaction conditions on its catalytic profile using three different C18 PUFA substrates. The results demonstrated that Bt-LOX exhibits significant HPI activity, especially at high enzyme concentrations, with ketone formation showing strong substrate dependence. Oxygen level was identified as a critical factor in directing the catalytic performance of Bt-LOX: HPI activity was inhibited under O₂-saturated conditions and enhanced under O₂-limited conditions. These findings establish Bt-LOX as the first bacterial LOX reported to exhibit pronounced HPI activity, and highlights its expanded potential for biocatalytic applications.

Item Type:	Article
Uncontrolled Keywords:	Epoxy alcohol; Hydroperoxide; Hydroperoxide isomerase; Ketone; Lipoxygenase
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QR Microbiology
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	RUTH CHRISNASARI - 210001
Date Deposited:	15 Aug 2025 08:28
Last Modified:	15 Aug 2025 08:28
URI:	http://repository.ubaya.ac.id/id/eprint/49418

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