Bacterial lipoxygenases for fatty acid oxyfunctionalization: advances and future directions

Chrisnasari, Ruth and Ewing, Tom A. and van Berkel, Willem J. H. and Vincken, Jean-Paul and Hennebelle, Marie (2026) Bacterial lipoxygenases for fatty acid oxyfunctionalization: advances and future directions. Applied Microbiology and Biotechnology, 110 (197). pp. 36-51. ISSN 0175-7598; E-ISSN 1432-0614

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Abstract

The shift to a biobased economy aims to reduce dependence on fossil fuels by converting biological materials, such as plant- and algae-derived fatty acids, into valuable compounds. The enzymatic oxyfunctionalization of fatty acids is of special interest because it enables the eco-friendly synthesis of a wide range of products with unique properties. Lipoxygenases (LOXs) are key oxyfunctionalization enzymes featuring broad substrate specificities and distinct regioselectivities. Among them, bacterial LOXs remain underexplored compared to their eukaryotic counterparts, representing an untapped resource with unique structural and functional traits. This review highlights recent advances in bacterial LOXs, emphasizing their versatile oxidative capabilities via dioxygenase and hydroperoxide isomerase activities. Key strategies, including protein engineering and oxygen level modulation, are discussed for modifying substrate specificity, regioselectivity, and preferred catalytic pathway. Multi-enzyme cascade reactions further expand the scope of LOX-derived products, unlocking new applications in biotechnological contexts. We address challenges in harnessing bacterial LOXs’ biocatalytic potential and propose solutions to optimize their performance. This work enhances understanding of bacterial LOXs and highlights their potential for transformative biotechnological applications in fatty acid derivatization.

Item Type:	Article
Uncontrolled Keywords:	Dioxygenase; Hydroperoxide isomerase; Lipoxygenase; Oxyfunctionalization; Oleochemicals; Oxidoreductase; Oxylipins
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	RUTH CHRISNASARI - 210001
Date Deposited:	07 Jul 2026 03:31
Last Modified:	07 Jul 2026 03:33
URI:	http://repository.ubaya.ac.id/id/eprint/50913

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