Thermal Denaturation Study on The Binding of A Methylene Blue Derivative to A Triple Helix Forming Oligonucleotide

Purwanto, Maria Goretti Marianti and Weisz, Klaus (2013) Thermal Denaturation Study on The Binding of A Methylene Blue Derivative to A Triple Helix Forming Oligonucleotide. International Journal of Applied Chemistry, 9 (3). pp. 253-267. ISSN 0973-1792

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Photoactive methylene blue(MB) is a cationic planar chromophore which is known to interact with double helical and triple helical DNA. UV spectroscopy have been employed to investigate the interaction between this dye and various DNA sequences, thus complementing the indirect data from LD, CD and/or fluorescence spectroscopic data available up to now. Covalent attachment of the MB to the DNA through a flexible heptamethylene linker has been accomplished in several steps, enabling the defined positioning of the dye moiety at specific sites of the DNA strands. UV thermal melting experiments indicate no significant stabilization/destabilization, demonstrated that MB interacts with all triplexforming DNA sequences studied here, albeit in different binding modes and strengths. In contrast, for sequences with the duplex overhang moved to the other side of the construct and with the MB moiety facing the terminal T-A.T triplex tract, imino resonances were noticeably sharper compared to the identical triplex sequence without MB. The corresponding triple helix-MB conjugate was stabilized through the dye-DNA interactions by up to IO°C as indicated by the UV melting data....file abstrak

Item Type: Article
Uncontrolled Keywords: UV Thermal Denaturation, Methylene Blue, DNA-ligand interaction
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Technobiology > Department of Biology
Depositing User: Eko Setiawan 194014
Date Deposited: 19 Dec 2013 08:33
Last Modified: 15 Mar 2021 15:43

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