Enzymatic Hydrolysis of Alkaline Pretreated Coconut Coir

Fatmawati, Akbarningrum and Agustriyanto, Rudy and Liasari, Yusnita (2012) Enzymatic Hydrolysis of Alkaline Pretreated Coconut Coir. In: 1st International Conference on Chemical Engineering and Material Engineering (ICCME) 2012, 12-13 September 2012, Univeristas Diponegoro.

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Abstract

The purpose of this research is to study the effect of concentration and temperature on the cellulose and lignin content, and the reducing sugars produced in the enzymatic hydrolysis of coconut coir. In this research, the coconut coir is pretreated using 3%, 7%, and 11% NaOH solution at 60oC, 80oC, and 100oC. The pretreated coir were assayed by measuring the amount of cellulose and lignin and then hydrolysed using Celluclast and Novozyme 188 under various temperature (30oC, 40oC, 50oC) and pH (3, 4, 5). The hydrolysis results were assayed for the reducing sugar content. The results showed that the alkaline delignification was effective to reduce lignin and to increase the cellulose content of the coir. The best delignification condition was observed at 11% NaOH solution and 100oC which removed 14,53% of lignin and increased the cellulose content up to 50,23%. The best condition of the enzymatic hydrolysis was obtained at 50oC and pH 4 which produced 7,57 gr/L reducing sugar.

Item Type:	Conference or Workshop Item (Lecture)
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Engineering > Department of Chemical Engineering
Depositing User:	Akbarningrum F, 61115
Date Deposited:	18 Sep 2012 07:45
Last Modified:	18 Sep 2012 07:45
URI:	http://repository.ubaya.ac.id/id/eprint/1107

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