Functional characterization of the pathogenesis-related protein family 10 gene, PgPR10-4, from Panax ginseng in response to environmental stresses

Kim, Yu-Jin and Jang, Moon-Gi and Lee, Hye-Jin and Jang, Gyu-Hwan and Sukweenadhi, Johan and Kwon, Woo-Saeng and Yang, Deok-Chun (2014) Functional characterization of the pathogenesis-related protein family 10 gene, PgPR10-4, from Panax ginseng in response to environmental stresses. Plant Cell, Tissue and Organ Culture (PCTOC), 118 (3). pp. 531-543. ISSN 0167-6857 (print), 1573-5044 (online)

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Abstract

Pathogenesis-related proteins (PRs) are known to function in higher plants as a protein-based defensive system against abiotic and biotic stress, particularly pathogen infections. A full-length cDNA sequence of PR BetV1 was isolated and characterized from a 14-year-old ginseng expressed sequence tags library and we named this as PgPR10-4, because of similar identities with previous isolated PgPR10s sequences. The PgPR10-4 gene encodes a 477 bp open reading frame and its deduced protein contains 158 amino acids with a 53 % identity with that of the Actinidia chinensis BetV1 allergen. The expression of PgPR10-4 gene was abundant in leaves and its transcripts showed differentially up-regulated patterns against several ginseng pathogens and abiotic stimuli such as high light and salinity. In addition, PgPR10-4 expression was strongly responsive towards the stress signaling molecules H2O2 and jasmonic acid (JA), while weekly responsive to salicylic acid and abscisic acid. A functional role of PgPR10-4 in environmental stress tolerance was further validated through its overexpression in Arabidopsis. An analysis of T2 transgenic Arabidopsis plants overexpressing the PgPR10-4 gene showed an enhanced tolerance to bacterial and fungal infection, but not to salt stress. When we tagged with cyan fluorescent protein fusion protein, the PgPR10-4-was found to localize to the cytoplasm. The enhanced antifungal activity observed from the Arabidopsis transgenic lines suggests the possible involvement of PgPR10-4 in a defense-related mechanism via the JA signaling pathway.

Item Type:	Article
Uncontrolled Keywords:	BetV1 protein , Biotic stress , Panax ginseng , Pathogenesis-related protein
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	JOHAN SUKWEENADHI
Date Deposited:	22 Nov 2017 05:03
Last Modified:	22 Nov 2017 06:03
URI:	http://repository.ubaya.ac.id/id/eprint/31174

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