Karakterisasi Senyawa Metabolit pada Kultur Anggrek Dendrobium anosmum-gigantea

Krisnawan, Alfian Hendra and Palupi, Sajekti and Wandhini, Ni Made Swasti and Suhartina, Sitti and Saputri, Alrivani Eka Yuli and Putri, I Gusti Agung Made Shinta Ari and Yunita, Oeke (2020) Karakterisasi Senyawa Metabolit pada Kultur Anggrek Dendrobium anosmum-gigantea. Media Pharmaceutica Indonesiana, 3 (1). pp. 10-18. ISSN 2527-9017

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Abstract

Dendrobium sp. is one of the orchid species that was used as a medicinal plant and produces a variety of important metabolites such as alkaloids having antioxidant, anticancer, and neuroprotective activity. This research was aimed to analyze the composition of metabolites produced from shoot culture of Dendrobium anosmum-gigantea compared with related parent plants using phytochemical screening, chromatographic, and densitometric methods. Shoot cultures were performed using MS medium with 0.5 ppm NAA and 0.5 ppm BAP. The extraction of shoot cultures and parent plants was carried out by ultrasonication. Phytochemical screening was conducted using the spot test method and Thin Layer Chromatography (TLC). The results showed that the shoot cultures and parent plant extracts contain four chemical compound groups, i.e. alkaloids, flavonoids, tannins, and phenols. Alkaloid analysis on shoot cultures and parent plants by TLC method using the mobile phase of chloroform : methanol (12 : 2) showed two spots at the Rf value of 0.41-0.43 and 0.75-0.78. Densitogram showed two peaks at the Rf value of 0.50-0.60 and 0.90-1.00. In conclusion, shoot culture of Dendrobium anosmum-gigantea and its parent plant showed similar compounds, therefore shoot culture can be used as an alternative for the Dendrobium anosmum-gigantea parent plant.

Item Type:	Article
Uncontrolled Keywords:	dendrobium; shoot culture; alkaloids; phytochemical screening
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	ALFIAN HENDRA KRISNAWAN
Date Deposited:	14 Sep 2020 13:16
Last Modified:	01 Sep 2021 06:39
URI:	http://repository.ubaya.ac.id/id/eprint/38091

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