Xylanase production from combined Reutealis trisperma with potato dextrose broth by Tricodherma reesei: the effect of pretreatment

Agustin, Yuana Elly and Riadi, Lieke and Utami, Titie Prapti (2019) Xylanase production from combined Reutealis trisperma with potato dextrose broth by Tricodherma reesei: the effect of pretreatment. In: International Conference on Informatics, Technology, and Engineering 2019 (InCITE 2019), 22-23 August 2019, Bali.

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Abstract

Xylanase is an important enzyme in pulp and paper, food, feed, and textile industries. It is produced by fermentation process with Tricodherma reesei as fungi, which grows on several carbon sources such as cellulose and xylan. Reutealis trisperma (kemiri sunan) have been used for biodiesel production after being mechanically pressed. The solid waste, which is called Reutealis trisperma cake (RTC) contains carbon and nitrogen sources which are valuable to be used for xylanase production by T. reesei. Shake flask systems were used to grow T. reesei in medium consists of RTC and Potato Dextrose Broth (PDB). The systems were maintained at 28oC, 200 rpm at initial pH of 6.0 for 84 hours. Two forms RTC used in this study were natural and alkaline pretreated. The RTC fermentations were performed for different concentration of PDB (15g/L and 20 g/L) and also for different concentration of RTC (13.33 g/L; 16.67 g/L and 20 g/L). Pretreated systems gave better yield of xylanase production compared to natural systems. Higher concentration of PDB for pretreated system was found to yield lower xylanase production. The highest xylanase activity (198.1 U/mL) was obtained in media containing 15 g/L PDB and 20 g/L pretreated RTC.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Engineering > Department of Chemical Engineering
Depositing User:	Lieke Riadi 6116
Date Deposited:	30 Oct 2020 04:02
Last Modified:	03 Sep 2021 02:43
URI:	http://repository.ubaya.ac.id/id/eprint/38355

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