G‐Quadruplex Formation in a Putative Coding Region of White Spot Syndrome Virus: Structural and Thermodynamic Aspects

Vianney, Yoanes Maria and Purwanto, Maria Goretti Marianti and Weisz, Klaus (2021) G‐Quadruplex Formation in a Putative Coding Region of White Spot Syndrome Virus: Structural and Thermodynamic Aspects. ChemBioChem, 22 (11). pp. 1932-1935. ISSN 1439-4227

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Abstract

White spot disease (WSD) is one of the most devastating viral infections of crustaceans caused by the white spot syndrome virus (WSSV). A conserved sequence WSSV131 in the DNA genome of WSSV was found to fold into a polymorphic G‐quadruplex structure. Supported by two mutant sequences with single G→T substitutions in the third G4‐tract of WSSV131, circular dichroism and NMR spectroscopic analyses demonstrate folding of the wild‐type sequence into a three‐tetrad parallel topology comprising three propeller loops with a major 1:3:1 and a minor 1:2:2 loop length arrangement. A thermodynamic analysis of quadruplex formation by differential scanning calorimetry (DSC) indicates a thermodynamically more stable 1:3:1 loop isomer. DSC also revealed the formation of additional highly stable multimeric species with populations depending on potassium ion concentration.

Item Type:	Article
Uncontrolled Keywords:	G-quadruplexes, NMR spectroscopy, thermodynamics, white spot syndrome virus
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	Eko Setiawan 194014
Date Deposited:	15 Mar 2021 15:25
Last Modified:	12 Nov 2021 04:22
URI:	http://repository.ubaya.ac.id/id/eprint/39020

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