Novel Antiviral Investigation of Annona squamosa Leaf Extract against the Dengue Virus Type-2: In vitro Study

Ansori, Arif Nur Muhammad and Fadholly, Amaq and Proboningrat, Annise and Antonius, Yulanda and Hayaza, Suhailah and Susilo, Raden Joko Kuncoroningrat and Inayatillah, Bilqis and Sibero, Mada Triandala and Naw, Sin War and Posa, Gabrielle Ann Villar and Sucipto, Teguh Hari and Soegijanto, Soegeng (2021) Novel Antiviral Investigation of Annona squamosa Leaf Extract against the Dengue Virus Type-2: In vitro Study. Pharmacognosy Journal, 13 (2). pp. 456-462. ISSN 0975-3575

PDF PharmacognJ-13-2-456.pdf.pdf - Published Version Download (4MB)

Abstract

Introduction: Dengue virus (DENV) infection is general mosquito-transmitted viral taint. It can lead to the dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Dengue is a solemn illness with no endowed antiviral medication or recognized vaccine. Therefore, we aimed to investigate the activity of Annona squamosa leaf extract (ASLE) against dengue virus type-2 (DENV-2) isolated from Surabaya, Indonesia in 2013 (NCBI accession number: KT012509). Methods: In this study, the antiviral activity of ASLE was evaluated against DENV- 2 in Vero cells using Viral ToxGlo™ Assay. In addition, we used CellTiter-Glo® Luminescent Cell Viability Assay to set the amount of viable cells in culture based on quantitation of the ATP. Results: DENV-2 replication inhibited by ASLE in Vero cells with IC50 = 73.78 μg/mL and SI = 4.49 when cells were treated two days after virus infection, whereas its CC50 for cytotoxicity to Vero cells was 331.54 μg/mL. Interestingly, this is the first report on the investigation of ASLE against DENV-2. Conclusion: In summary, ASLE demonstrated the antiviral activity against DENV-2 with less toxicity, and high possibility as a drug candidate. Therefore, it might be suggested for in vivo assessment in the progress of a potent antiviral against DENV-2.

Item Type:	Article
Uncontrolled Keywords:	Annona squamosa, Antiviral activity, DENV-2, Vero cells
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General)
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	YULANDA ANTONIUS
Date Deposited:	09 Feb 2022 07:11
Last Modified:	09 Feb 2022 07:11
URI:	http://repository.ubaya.ac.id/id/eprint/41220

Actions (login required)

View Item