High-Fat Diet Decreases Serum TNF-Alpha and Breast Tumor Area on Benzopyrene Induced Mice (Mus Musculus)

Rejeki, Purwo Sri and Utami, Dita Mega and Izzatunnisa, Nabilah and Pranoto, Adi and Sukarno, Devitya Angielevi and Fasitasari, Minidian (2021) High-Fat Diet Decreases Serum TNF-Alpha and Breast Tumor Area on Benzopyrene Induced Mice (Mus Musculus). Chiang Mai University Journal of Natural Sciences, 20 (4). e2021089. ISSN 1685-1994

PDF Sukarno-High-Fat Diet Decreases Serum TNF-Alpha and Breast Tumor Area on Benzopyrene Induced Mice (Mus Musculus).pdf Download (6MB)

Abstract

This study aims to analyze whether the high-fat diet decreases serum TNF-α and breast tumor area on benzopyrene induced mice (Mus musculus). This study was a true experimental with the randomized posttest-only control group design using 36 female mice (Mus musculus), 3-4 months age, 25 ± 5 grams. Mice were induced with benzopyrene (BZP) subcutaneously with a dose of 0.3mg/20gBB/day for 14 days in the right breast area, then randomly divided into 6 groups, K1 (negative control group, given standard feed), K2 (positive control group, standard feed), K3 (high-fat diet with a ratio of 60% protein, 0% carbohydrate, 30% fat, 10% fiber), K4 (high-fat diet with a ratio of 45% protein, 0% carbohydrate, 45% fat, 10% fiber), K5 (high-fat diet with a ratio of 30% protein, 0% carbohydrate, 60% fat, 10% fiber) and K6 (high-fat diet on day 15 with a ratio of 15% protein, 0% carbohydrate, 75% fat, 10% fiber). The high-fat diet was administered for 28 days. The mean of tumor area delta at K1 (0.00 ± 0.00) mm2, K2 (3.52 ± 1.98) mm2, K3 (27.18 ± 21.23) mm2, K4 (13.19 ± 9.93) mm2, K5 (8.80 ± 1.72) mm2, K6 (10.81 ± 6.55) mm2, and (p=0.001). The mean of TNF-α levels at K1 (56.32 ± 8.25) ng/mL, K2 (65.99 ± 2.82) ng/mL, K3 (70.43 ± 4.61) ng/mL, K4 (58.05 ± 5.80) ng/mL, K5 (54.91 ± 3.27) ng/mL, K6 (59.67 ± 3.63) ng/mL and (P = 0.000). A high-fat diet lowers TNF-α levels and reduces the area of BZP-induced breast tumors. The lowest TNF-α levels and the lowest breast tumor area were found in groups with a fat: protein ratio = 60:30.

Item Type:	Article
Uncontrolled Keywords:	Benzopyrene induced, Breast tumor area, High-fat diet, Tumor necrosis factor-α
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medical
Depositing User:	Ester Sri W. 196039
Date Deposited:	20 Apr 2022 03:03
Last Modified:	20 Apr 2022 03:03
URI:	http://repository.ubaya.ac.id/id/eprint/41784

Actions (login required)

View Item