Ratih, Ratih and Stein, Matthias and Asmari, Mufarreh and Deeb, Sami El (2019) Protein-Based Affinity Capillary Electrophoresis for Enantioseparation of Calcium Channel Blockers. In: 26th lnternational Symposium on Electroseparation and Liquid Phase-Separation Techniques, ITP 2019-Toulouse, 1-4 September 2019, France.
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Abstract
The development of separation methods for chiral compounds has been an interesting field in pharmaceutical research, particularly the use of proteins as the binding agent. ln this study, the enantioselectivity of calcium channel blockers was investigated using affinity capillary electrophoresis, with human serum as a chiral selector. For this purpose, positively charged of racemic drugs, amlodipine and verapamil were selected as chiral drug models. As the sample was introduced in the capillary electrophoresis system, enantiomer bound to human serum albumin in different extents. Baseline separation was achieved in bare-fused silica capillary with a 20 mM phosphate buffer pH 7 .4, at 15 kV applied voltage and 25 oC. The difference in apparent mobility shifts of enantiomers corresponds to the resolution value of 1.0-3.9. Association constant of the enantiomers was determined using nonlinear regression. R- (+)-verapamil performed to be bound stronger to serum albumin compared with S-(-)-verapamil. The Kn value of S- (-)-amlodipine in the racemic mixture was found to be higher than its antipode. ln addition to this, a close agreement with the S-(-)- enantiomer was achieved for amlodipine.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Uncontrolled Keywords: | enantioseparation, affinity capillary electrophoresis, human serum albumin, chiral selector |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Pharmacy > Department of Pharmacy |
| Depositing User: | Ester Sri W. 196039 |
| Date Deposited: | 05 Jun 2023 04:45 |
| Last Modified: | 05 Jun 2023 05:02 |
| URI: | http://repository.ubaya.ac.id/id/eprint/44233 |
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