Investigation of Enantioselective Interaction and Determination of Binding Constants of Two Calcium Channel Blockers using Capillary Electrophoresis

Ratih, Ratih and Stein, Matthias and Asmari, Mufarreh and Deeb, Sami El (2019) Investigation of Enantioselective Interaction and Determination of Binding Constants of Two Calcium Channel Blockers using Capillary Electrophoresis. In: 26 th lnternational Symposium on Electroseparation and Liquid Phase-Separation Techniques, ITP 2019-Toulouse, 1-4 September 2019, France.

PDF Ratih_Investigation of Enantioselective Interaction and Determination.pdf Download (5MB)

Abstract

The individual enantiomers of a racemic drug usually exhibit specific therapeutic efficacy. In order to achieve optimal therapeutic treatment, an enantioselective analytical method is needed for quality control. Capillary electrophoresis is an analytical method which shows high efficiency in enantiomeric separation. In this study, the enantioselectivity of two calcium channel blockers was investigated using chiral macromolecule as a selector in the background electrolyte of the capillary electrophoresis system. Polysaccharide-based and protein-based selectors were employed separately, each at different concentrations and conditions (e.g., pH value and applied voltage) to achieve baseline separation. Furthermore, the interaction of each enantiomer with human serum albumin as the most abundant protein in the biological system was studied. Mobility-shift affinity capillary electrophoresis was applied to perform the enantiomeric separation and the determination of binding constant. The apparent binding constant of each enantiomer was measured by nonlinear regression analysis. The R- and S- enantiomers of each drug model showed different binding constant values

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	enantioselective interaction, affinity capillary electrophoresis, human serum albumin, chiral selector
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	06 Jun 2023 02:12
Last Modified:	22 Nov 2023 03:16
URI:	http://repository.ubaya.ac.id/id/eprint/44239

Actions (login required)

View Item