α-Amylase and α-Glucosidase Inhibition Effect Of Several Indonesian Plants Exctract

Setiawan, Finna and Putri, Evi Novita and Wahyuni, Sri and Artikasari, Cindy and Yunita, Oeke and Jayani, Nikmatul Ikhrom Eka and Kartini, Kartini (2021) α-Amylase and α-Glucosidase Inhibition Effect Of Several Indonesian Plants Exctract. In: International Seminar on Traditional Herbal Medicine 2021 (ISTHM 2021), July 8-9th 2021, Bandar Lampung.

	PDF Fina Setiawan_α-Amylase and α-Glucosidase.pdf Download (540kB)
	PDF 4_ST-PM_FF_VII_2021.pdf Restricted to Registered users only Download (102kB) | Request a copy

Abstract

The aim of this research was to determine the inhibition activity of nine Indonesian plant extracts, i.e. turmeric (Curcuma longa), Javanese turmeric (Curcuma xanthorrhiza), sappan wood’s (Caesalpinia sappan), meniran (Phyllanthus niruri), cat whiskers (Orthosiphon stamineus), celery (Apium graveolens), tempuyung (Sonchus arvensis), kepel (Stelecocarpus burahol), and pegagan (Centella asiatica) against α-amylase and α- glucosidase enzymes. All of crude drugs were extracted with soxhlet apparatus using 80% ethanol. α-amylase and α-glucosidase inhibition assays were done in vitro using Sigma Aldrich method with several modifications. The result showed that meniran, sappan wood’s, Javanese turmeric, turmeric, kepel, cat whiskers, celery, pegagan, and tempuyung extracts could inhibit the activity of α-glucosidase enzyme with IC50 values <20, 37.36, 222.24, 285.94, 291.25, 354.61, 835.72, >1000, and > 1000 μg/ml, respectively. Moreover, IC50 value of sappan wood’s and meniran extracts on α-amylase enzyme were 317.31 and 496.71 μg/ml, respectively. At the other hand, turmeric, Javanese turmeric, tempuyung, kepel, cat whiskers, and pegagan extracts exhibited same IC50 value on this enzyme, i.e. >1000 μg/ml. In Conclusion, meniran has the best inhibition activity of an α-glucosidase enzyme. At the same time, sappan wood’s has the best inhibition activity of an α-amylase enzyme.

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	α-amylase assay, α-glucosidase assay, Indonesian plants
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	26 Jun 2023 04:03
Last Modified:	26 Jun 2023 04:08
URI:	http://repository.ubaya.ac.id/id/eprint/44372

Actions (login required)

View Item