Ekstrak Kasar Isolat Bacillus sp Sebagai Agen Peluruh Biofilm

Goeltom, Mangihot Tua and Thamrin, William and Wahjudi, Mariana and Suhartono, Suhartono (2023) Ekstrak Kasar Isolat Bacillus sp Sebagai Agen Peluruh Biofilm. Keluwih: Jurnal Kesehatan dan Kedokteran, 4 (2). pp. 8-15. ISSN 2715-6419

PDF Mangihot Tua Gultom_Ekstrak Kasar Isolat Bacillus sp.pdf Download (2MB)

Abstract

Biofilms play an important role in the distribution and pathogenicity of bacteria. In our previous research, we demonstrated that our isolated Bacillus sp. (collection of Microbiology Lab, Universitas Surabaya) exhibited in vitro antibiofilm activity. Bacilli generally produce cellulase, which is one of the enzymes responsible for bacterial biofilm degradation. Based on this fact, this study aimed (1) to determine whether or not the crude extract of our Bacillus sp. isolate is able to degrade biofilms formed by Escherichia coli, Staphylococcus aureus, and water-crane bacteria, and (2) to determine whether such crude extract contains active cellulase. The research steps began with the preparation of Bacillus sp. crude extract, biofilm degradation assay, cellulase activity assay on CMC Agar, and cellulase detection by SDS-PAGE and electrophoresis zymogram. The crude extract of Bacillus sp. effectively degraded the biofilm 30 min after contact. The crude extract decreased biofilms of Escherichia coli 23,56%, Staphylococcus aureus 27,78%, and water-crane bacteria 37,98%. Based on the zymogram result, it was shown that the crude extract contained active cellulase, with a size of approximately 35 kDA. It was concluded that the crude extract of Bacillus sp. has potential as an anti-biofilm agent and exhibits active cellulase activity.

Item Type:	Article
Uncontrolled Keywords:	Bacillus sp., crude extract, biofilm, SDS-PAGE, zymogram
Subjects:	T Technology > T Technology (General)
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	Ester Sri W. 196039
Date Deposited:	30 Aug 2023 03:20
Last Modified:	13 Oct 2023 03:34
URI:	http://repository.ubaya.ac.id/id/eprint/44878

Actions (login required)

View Item