hWJMSCs inhibit inflammation and apoptosis in an ARDS cell model

Widowati, Wahyu and Wargasetia, Teresa L. and Rahardja, Fanny and Gunanegara, Rimonta F. and Priyandoko, Didik and Gondokesumo, Marisca Evalina and Novianto, Agung and Yati, Afif and Rizal, Rizal (2023) hWJMSCs inhibit inflammation and apoptosis in an ARDS cell model. Journal of Taibah University Medical Sciences, 18 (6). pp. 1519-1526. ISSN 1658-3612

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Abstract

Acute respiratory distress syndrome (ARDS) is a type of lung failure caused by fluids and hypoxemia. Mesenchy- mal stem cells (MSCs) have been shown to decrease levels of pro-inflammatory mediators and inflammatory cells. These cells have anti-inflammatory, anti-apoptotic, and anti-microbial activity, and protect against lung injury.Objective: This research evaluated the potential of hu- man Wharton’s jelly MSCs (hWJMSCs) to inhibit inflammation and apoptosis in lipopolysaccharide (LPS)- induced rat lung cells (L2). Methods: hWJMSC treatment in LPS-induced rat lung cells was performed with 1:1, 1:5, 1:10, or 1:25 ratios of hWJMSCs to L2 cells. The gene expression of angiotensin-converting enzyme-2 (ACE-2), receptor for advanced glycation end products (RAGE), nuclear factor kappa B (NFkB), and C-X-C motif chemokine ligand-9 (CXCL-9) was quantified with RT-PCR, and the levels of C-reactive protein (CRP), interleukin-12 (IL-12), and tumor necrosis factor-alpha (TNF-a) were measured with ELISA. Results: hWJMSCs increased ACE-2 gene expression, and decreased CXCL-9, NFkB, and RAGE gene expression. The treatment also suppressed CRP, TNF-a, and IL-12 levels, and increased the percentage of live cells, but decreased the percentages of necrotic cells and apoptotic cells in inflammatory rat lung cells, which served as an ARDS cell model. Conclusion: Co-culture of hWJMSCs and L2 cells miti- gated inflammation through increasing ACE-2 gene expression, and decreasing CXCL-9, NFkB, and RAGE gene expression; decreasing TNF-a and CRP protein levels; and decreasing necrosis, and early and late apoptosis. A co-culture ratio of 1:1 was most effective

Item Type:	Article
Uncontrolled Keywords:	Apoptosis; ARDS; hWJMSCs; Inflammation; NFkB
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	30 Jul 2024 08:16
Last Modified:	30 Jul 2024 22:44
URI:	http://repository.ubaya.ac.id/id/eprint/46796

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