Phyllanthus niruri leaves: Unveiling their reversible anti-inflammatory activity on MIF protein and macrophage cells

Kok, Tjie and Juniansa, Angga and Surtikanthi, Dewi Galuh and Irawati, Fenny and Guan, Zheng (2025) Phyllanthus niruri leaves: Unveiling their reversible anti-inflammatory activity on MIF protein and macrophage cells. Bioactive Compounds in Health and Disease, 8 (8). pp. 308-317. ISSN 2769-2426; E-ISSN 2574-0334

PDF Phyllanthus niruri leaves.pdf Download (554kB)

Abstract

Background: Phyllanthus niruri leaves have been known for their various biological activities, including potential anti-inflammatory effects for prevention and treatment of chronic diseases. However, their anti-inflammatory effects have not yet been comprehensively elaborated. This study introduces the novel finding that Phyllanthus niruri leaf extract reversibly exhibits inhibition of macrophage migration inhibitory factor (MIF) activity, a key protein in chronic inflammation, with a low IC50. Furthermore, it demonstrates the extract's ability to reduce the levels of pro-inflammatory cytokines IL-1β and IFN-γ in macrophage cells, suggesting a previously unelaborated mechanism for its anti-inflammatory potential. Methods: The research innovatively combines the assessment of MIF tautomerase inhibition with the evaluation of pro-inflammatory cytokine reduction in macrophage cells to comprehensively characterize the anti-inflammatory activity of Phyllanthus niruri. The determination of the reversibility of MIF inhibition adds a crucial layer of understanding to its potential therapeutic action, suggesting a dynamic interaction with the target protein. Results: Phyllanthus niruri leaf extract inhibited MIF activity reversibly with an IC50 in the low mg/L range and reduced the concentrations of IL-1β and IFN-γ pro-inflammatory cytokines in macrophage RAW 264.7 cells to different extents. Conclusion: These findings suggest that Phyllanthus niruri leaf extract holds promise as a natural anti-inflammatory agent for managing inflammation-associated chronic diseases. The demonstrated in vitro efficacy warrants further investigation into its potential as a therapeutic or preventive agent, paving the way for future in vivo studies and clinical trials to validate its use in human health.

Item Type:	Article
Uncontrolled Keywords:	inflammatory-associated chronic diseases, macrophage migration inhibitory factor (MIF), macrophage cells, Phyllanthus niruri, anti-inflammatory agent
Subjects:	T Technology > T Technology (General)
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	Ester Sri W. 196039
Date Deposited:	02 Sep 2025 03:44
Last Modified:	02 Sep 2025 03:44
URI:	http://repository.ubaya.ac.id/id/eprint/49485

Actions (login required)

View Item