Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model

Widowati, Wahyu and Wargasetia, Teresa Liliana and Rahardja, Fanny and Gunanegara, Rimonta F. and Priyandoko, Didik and Gondokesumo, Marisca Evalina and Afifah, Ervi and Wijayanti, Cahyaning Riski and Rizal, Rizal (2022) Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model. Asian Pacific Journal of Tropical Biomedicine, 12 (8). pp. 343-350. ISSN 2221-1691;E-ISSN 2588-9222

PDF Human Wharton’s jelly.pdf Download (4MB)

Abstract

Objective: To evaluate the potential effect of human Wharton’s jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats. Methods: The hWJMSCs (5×104/mL, 5×105/mL, 5×106/mL) were administered to rats on day 1 and day 8 after being induced by LPS (5 mg/kg body weight). TNF-α levels in the lung and IL- 18 and IL-1β levels in the serum were measured using ELISA. In addition, caspase-1 expression in lung tissues was quantified using qRT-PCR, and NF-κB and IL-6 expressions were assessed using immunohistochemistry. Results: The hWJMSCs decreased TNF-α levels in the lung and plasma IL-18 and IL-1β levels. Moreover, the hWJMSCs downregulated the expressions of caspase-1, IL-6, and NF-κB in lung tissues. Conclusions: The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome.

Item Type:	Article
Uncontrolled Keywords:	Human Wharton’s jelly mesenchymal stem cells; Acute respiratory distress syndrome; TNF-α; IL-18; NF-κB; Inflammatory marke
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	Ester Sri W. 196039
Date Deposited:	11 Feb 2026 03:04
Last Modified:	11 Feb 2026 03:04
URI:	http://repository.ubaya.ac.id/id/eprint/50268

Actions (login required)

View Item