Pengembangan Metode Ekstraksi dan Analisis Irbesartan dalam Plasma Darah menggunakan High Performance Liquid Chromatography (HPLC) Fluorescence

Ratih, Ratih (2015) Pengembangan Metode Ekstraksi dan Analisis Irbesartan dalam Plasma Darah menggunakan High Performance Liquid Chromatography (HPLC) Fluorescence. Jurnal Farmasi Indonesia, 7 (3). pp. 157-162. ISSN 1412-1107

Preview	PDF Ratih_Pengembangan Metode_Abstrak_2015.pdf Download (678kB) | Preview
	PDF Ratih_Pengembangan Metode_2015.pdf Restricted to Repository staff only Download (492kB) | Request a copy

Abstract

A simple extraction and analysis method by High Performance Liquid Chromatography (HPLC) Fluorescence has developed for determination of Irbesartan in plasma sample. The chromatography system were Fluorescence detector at λ ex254nm/em380 nm, Flow rate 1 mL/min, C-18 150 x 4,6 mm; 5μm column and acetonitrile : 0,5% (v/v) H3PO4 pH 3,0 (77:23) as mobile phase by run time not more than 6 minutes. Reliability of the HPLC method was assessed by the linearity of 9 levels concentration which be found 10.0 – 5,000.0 ng/mL, r = 0.9996 respectively. Chloroform was used as pretreatment solvent to extract Irbesartan dan Candesartan cilexetil (Internal Standard) from protein binding. The chromatogram of Irbesartan dan Candesartan cilexetil peaks were clearly separated without matrix noises, it was showed by resolution factor > 2.

Item Type:	Article
Uncontrolled Keywords:	irbesartan, candesartan, HPLC Fluorescence, blood plasma
Subjects:	Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmacy > Department of Pharmacy
Depositing User:	RATIH
Date Deposited:	14 Jan 2016 03:18
Last Modified:	15 Dec 2023 04:51
URI:	http://repository.ubaya.ac.id/id/eprint/26515

Actions (login required)

View Item