Exploring diet‑induced promoter hypomethylation and PDK4 overexpression: implications for type 2 diabetes mellitus

Putra, Sulistyo Emantoko Dwi and Wijono, Heru and Sulistomo, Hikmawan Wahyu and Humardani, Farizky Martriano and Tanaya, Lady Theresa Adeodata and Mulyanata, Lisa Thalia and Kesuma, Dini and Ikawaty, Risma (2023) Exploring diet‑induced promoter hypomethylation and PDK4 overexpression: implications for type 2 diabetes mellitus. Molecular Biology Reports, 7 (23). pp. 8949-8958. ISSN 1573-4978

PDF Dini Kesuma_Exploring diet‑induced promoter hypomethylation_Rev.pdf Restricted to Registered users only Download (7MB) | Request a copy

Abstract

Background Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by limited metabolic flexibility in the body. Such limitation implicates the pyruvate dehydrogenase kinase 4 (PDK4) gene Poor nutrition, frequently observed among Southeast Asians usually involves excessive intakes of carbohydrates and monosodium glutamate (MSG), which have been frequently linked to an increased risk of T2DM. Methods The 14-week study aimed to assess the effects of high-carbohydrate (HC), high-MSG (HMSG), and a combination of high-carbohydrate and high-MSG (HCHMSG) diets on the development of T2DM using male mice. To assess the effects, the male mice were divided into four groups: control (C), HC, HMSG, and HCHMSG for 14 weeks. Results After 14 weeks, both the HC and HCHMSG groups showed signs of T2DM (168.83 ± 32.33; 156.42 ± 32.46). The blood samples from the HMSG, HC, and HCHMSG groups (57.67 ± 2.882; 49.22 ± 7.36; 48.9 ± 6.43) as well as skeletal muscle samples from the HMSG, HC, and HCHMSG groups (57.78 ± 8.54; 42.13 ± 7.25; 37.57 ± 10.42) exhibited a gradual hypomethylation. The HC groups particularly displayed significant PDK4 gene expression in skeletal muscle. A progressive overexpression of the PDK4 gene was observed as well in the HMSG, HCHMSG, and HC groups (2.03 ±3.097; 3.21 ±2.94; 5.86 ± 2.54). Conclusions These findings suggest that high-carbohydrate and high-MSG diets can induce T2DM. However, the sole consumption of high MSG did not lead to the development of T2DM. Further research should focus on conducting long-term studies to fully comprehend the impact of a high MSG diet on individuals with pre-existing T2DM.

Item Type:	Article
Uncontrolled Keywords:	Diet; Expression; Methylation; Pyruvate dehydrogenase kinase 4; Type 2 diabetes mellitus
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	Sulistyo Emantoko 61116
Date Deposited:	10 Nov 2023 02:37
Last Modified:	01 Apr 2024 05:38
URI:	http://repository.ubaya.ac.id/id/eprint/45294

Actions (login required)

View Item