Gene Expression Analysis of ERF15, WRKY11, and CYP450 in Tobacco Infected with Ralstonia solanacearum

Sukweenadhi, Johan and Setiabudi, James and Savitri, Wina Dian and Kang, Se Chan (2025) Gene Expression Analysis of ERF15, WRKY11, and CYP450 in Tobacco Infected with Ralstonia solanacearum. Sarhad Journal of Agriculture, 39 ((1)). pp. 84-90. ISSN 1016-4383, 2224-5383

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Abstract

Bacterial wilt is one of the destructive infectious diseases in tobacco plants (Nicotiana tabacum L.). Ralstonia solanacearum Rs is the bacteria responsible for the incidence of the disease. Crop yields can be dramatically reduced in quantity and quality due to attack by this pathogen. Developing cultivars resistant to Rs is a challenge to overcome this problem. Marker-assisted selection (MAS) plays a critical role as a selection tool in the development of high-yielding cultivars. The current study aims to provide basic information regarding the expression of several genes associated with Rs in tobacco. Four tobacco cultivars, namely Hick Broadleaf, Beinhart-1000, Dark-302, and Dark-314, were artificially infected using Rs and subjected to cDNA analysis using end-point PCR in triplication. Results showed that ERF15, WRKY11, and CYP450 genes showed no change in expression in the tested tobacco cultivars. Further experiments considering these factors could help elucidate the precise mechanisms governing the three genes expression in response to R. solanacearum infection.

Item Type:	Article
Uncontrolled Keywords:	Bacterial wilt, Biotic stress, Marker-assisted selection, Resistant cultivar, Tobacco, Virulent
Subjects:	Q Science > QK Botany Q Science > QR Microbiology S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	JOHAN SUKWEENADHI
Date Deposited:	20 May 2025 05:26
Last Modified:	21 May 2025 04:38
URI:	http://repository.ubaya.ac.id/id/eprint/48520

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