Valorization of Keting fish (Mystus nigriceps) viscera using papain for antioxidant protein hydrolysate production

Tirtawijaya, Gabriel and Christine, Evelyn and Putra, Sulistyo Emantoko Dwi and Askitosari, Theresia Desy (2025) Valorization of Keting fish (Mystus nigriceps) viscera using papain for antioxidant protein hydrolysate production. In: IOP Conference Series: Earth and Environmental Science,Volume 1478, IBC-9, ISIBio-11 and NRLS-5, 11/09/2024 - 14/09/2024, Malang.

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Abstract

Underutilized viscera from keting fish (Mystus nigriceps) processing in Kenjeran, Surabaya offer opportunities for resource recovery and pollution prevention. This study aimed to optimize the hydrolysis conditions of keting viscera using papain and to evaluate its potential as a source of antioxidant protein hydrolysate. The optimal hydrolysis conditions were determined based on the most effective scavenging activity of 2,2-diphenyl-1-picrylhydrazil (DPPH) radical. Viscera were hydrolyzed under various conditions, including four enzyme concentration levels (0, 1, 3, and 5%) and four hydrolysis durations (1, 2, 4, and 6 hours). Enzyme concentration, hydrolysis duration, and their interaction showed a significant influence on antioxidant activity (p < 0.05). Hydrolysate was produced under optimal conditions with a 5% papain solution applied for six hours. This resulted in a product with strong antioxidant properties, as measured by its IC50 value of 1.22 mg/mL against DPPH radicals. The proximate analysis of the hydrolysate showed the protein content was 30.04% and distributed its molecular weight in the range of 68 – 134 kDa and ≤ 10 kDa. This research demonstrates the feasibility of valorizing fish processing waste into a valuable product with prospective uses in the functional food, nutraceutical and pharmaceutical fields.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry S Agriculture > S Agriculture (General) S Agriculture > SH Aquaculture. Fisheries. Angling T Technology > TA Engineering (General). Civil engineering (General) T Technology > TD Environmental technology. Sanitary engineering
Divisions:	Faculty of Technobiology > Department of Biology
Depositing User:	GABRIEL TIRTAWIJAYA
Date Deposited:	28 Apr 2025 08:28
Last Modified:	27 May 2025 06:03
URI:	http://repository.ubaya.ac.id/id/eprint/48432

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